{"id":1371,"date":"2024-07-16T13:02:24","date_gmt":"2024-07-16T11:02:24","guid":{"rendered":"https:\/\/parviruslab1.unimi.it\/?page_id=1371"},"modified":"2024-09-11T16:28:16","modified_gmt":"2024-09-11T14:28:16","slug":"laboratory-techniques","status":"publish","type":"page","link":"https:\/\/parvirlab.unimi.it\/en\/research\/laboratory-techniques\/","title":{"rendered":"Laboratory techniques"},"content":{"rendered":"\n
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Molecular Biology<\/mark><\/h2>\n\n\n\n
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  • DNA\/RNA and protein extraction from various matrices, biological fluids, and cell lines (using manual column-based and automated methods)<\/li>\n\n\n\n
  • PCR and Real-Time PCR (SYBR Green and TaqMan)<\/li>\n\n\n\n
  • Electrophoresis (agarose gel, two-dimensional SDS-PAGE, and Western blot)<\/li>\n\n\n\n
  • Sequence analysis<\/li>\n\n\n\n
  • Molecular cloning techniques (insertion of constructs\/DNA fragments into plasmids)<\/li>\n\n\n\n
  • Sorting of mononuclear cells with magnetic beads<\/li>\n\n\n\n
  • Bacterial cultures in solid and liquid media \u2013 isolation and characterization of bacterial species<\/li>\n<\/ul>\n<\/div>\n\n\n\n
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    Cellular Biology<\/mark><\/h2>\n\n\n\n
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    • Expansion and maintenance of primary and immortalized cell cultures<\/li>\n\n\n\n
    • Co-cultures for autologous lymphocyte stimulation<\/li>\n\n\n\n
    • Stable and transient transfections \/infections of cell lines<\/li>\n\n\n\n
    • Use of reporter genes (luciferase) in immortalized cell lines<\/li>\n\n\n\n
    • Cell viability assays (MTT assay, LDH assay)<\/li>\n\n\n\n
    • Isolation and counting of mononuclear cells from peripheral blood using Ficoll technique<\/li>\n\n\n\n
    • Tubulogenesis assay on collagen matrix<\/li>\n\n\n\n
    • Immunomodulation assays, cytokine production (ELISA)<\/li>\n\n\n\n
    • Imaging techniques, immunofluorescence<\/li>\n<\/ul>\n<\/div>\n\n\n\n
      \n
      \"\"<\/figure>\n\n\n\n

      Parasitology and Virology<\/mark><\/h2>\n\n\n\n
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      • Long-term in vitro<\/em> culture of P. falciparum<\/em> and Leishmania<\/em> spp.<\/li>\n\n\n\n
      • Production of P. falciparum <\/em>gametocytes<\/li>\n\n\n\n
      • Chemosensitivity assays on P. falciparum<\/em> (asexual stage and gametocytes) and Leishmania<\/em> spp.<\/li>\n\n\n\n
      • Generation of transgenic Leishmania <\/em>spp. strains<\/li>\n\n\n\n
      • Viral titration using molecular (qRT-PCR) and cellular methods (plaque assay and TCID)<\/li>\n\n\n\n
      • Isolation of viruses from human samples such as nasal swabs, bronchoalveolar lavage<\/li>\n\n\n\n
      • Neutralization assays to evaluate the anti-SARS-CoV-2 activity of patient sera<\/li>\n<\/ul>\n<\/div>\n<\/div>\n","protected":false},"excerpt":{"rendered":"

        Molecular Biology Cellular Biology Parasitology and Virology<\/p>\n","protected":false},"author":319,"featured_media":0,"parent":1561,"menu_order":8,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_acf_changed":false,"footnotes":""},"class_list":["post-1371","page","type-page","status-publish","hentry"],"acf":[],"_links":{"self":[{"href":"https:\/\/parvirlab.unimi.it\/en\/wp-json\/wp\/v2\/pages\/1371"}],"collection":[{"href":"https:\/\/parvirlab.unimi.it\/en\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/parvirlab.unimi.it\/en\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/parvirlab.unimi.it\/en\/wp-json\/wp\/v2\/users\/319"}],"replies":[{"embeddable":true,"href":"https:\/\/parvirlab.unimi.it\/en\/wp-json\/wp\/v2\/comments?post=1371"}],"version-history":[{"count":3,"href":"https:\/\/parvirlab.unimi.it\/en\/wp-json\/wp\/v2\/pages\/1371\/revisions"}],"predecessor-version":[{"id":1688,"href":"https:\/\/parvirlab.unimi.it\/en\/wp-json\/wp\/v2\/pages\/1371\/revisions\/1688"}],"up":[{"embeddable":true,"href":"https:\/\/parvirlab.unimi.it\/en\/wp-json\/wp\/v2\/pages\/1561"}],"wp:attachment":[{"href":"https:\/\/parvirlab.unimi.it\/en\/wp-json\/wp\/v2\/media?parent=1371"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}